Compared to the conventional single-nozzle emitters, the M3 emitter from Newomics provides 3-5 times higher peptide signals in ESI-MS and more robust and stable spray across the LC gradient. This allows me to use microliter flow rate LC-MS for a large set of biological samples for biomarker discovery, and ensures the reproducible results and similar sensitivity as nanoLC-MS has.

The 8-nozzle emitter showed a 5-10-fold increase in sensitivity for protein therapeutics in human serum compared to the standard LC-MS (HESI source) at the flow rate of 5 μL/min. Preliminary analysis of replicate injections from the same samples showed an excellent CV by the measurement of peak area ratios and no additional carryover.

3X improvement in peak intensities was observed for the 8-nozzle emitter in comparison with the ESI sprayer for non-denaturing LC-MS. Moreover, the generally sharper peaks and charge envelope shifted towards higher m/z suggest a more nanospray-like emission and possibly better preservation of the native conformation.

From my tests, the detection of a mix of 10 peptides spiked in plasma extracts, was 5-6X more sensitive (and up to > 10X) with Newomics multi-nozzle emitter than with classical ESI source with a single-nozzle.