Newomics MnESI-MS platform streamlines analysis of native antibodies, antibody drug conjugates, and large protein complexes. It achieves static nanoflow sensitivity and excellent data quality, allows for automated processing and higher throughput via LC/MS, and provides better preservation of protein native state during the ESI process.

SEC-LC/MS analysis of GroEL tetradecaner protein complex using the MnESI-MS platform with M3 Emitter at the microflow of 5 µL/min.

Newomics MnESI-MS platform facilitates nucleic acid analysis in a negative mode. It delivers increased sensitivity and higher charge state which allows for detection of larger oligonucleotides with longer sequence, and better identification with more complete fragmentation (with a higher charge state).

Ion-Pair Reversed Phase (IP-RP) LC/MS Analysis of 75-mer tRNA using the MnESI-MS platform with M3 Emitter at microflow. MnESI platform attains improved sensitivity and higher charge state and comparable data quality vs. high-flow LC/MS method.

Newomics MnESI-MS platform accelerates bottom-up proteomics and quantitative proteomics. It attains nanoflow sensitivity, higher reproducibility, robustness, and throughput in the analysis of Hela cells and human plasma samples.

Comparison of sensitivity and reproducibility (peak area %RSD): MnESI-MS platform vs. nanoflow LC/MS performing PRM analysis of Promega peptide mixture spiked in 200 ng HeLa digest.

MnESI-MS platform with M3 emitter delivers > 40-fold sensitivity increase and comparable peak area reproducibility vs. conventional high flow LC/MS in targeted proteomics analysis of human plasma samples.

Newomics MnESI-MS platform allows for sensitive identification and quantitation of lipids in human plasma samples. It achieves increased sensitivity and assay reproducibility and allows hundreds of lipid species to be measured from a drop of blood.

MnESI-MS platform with M3 emitter achieves enhanced sensitivity (2.5-fold) vs. conventional nanoflow method using single nozzle emitters in shotgun lipidomics of human plasma samples.

Newomics Microflow-nanospray ESI-MS (MnESI-MS ) Platform

See the superior performance and features of our MnESI-MS platform based on Newomics award-winning multinozzle M3 emitter technology and the new MnESI source; and learn how the MnESI-MS platform benefits your LC/MS applications.
Brochure / English / Oct 2020

MnESI-MS Platform for LC-MS Analysis of Native Protein Complexes

This application note establishes a MnESI-MS platform for LC-MS analysis of labile native protein complexes, that achieves high throughput, sensitivity, and robustness, using the Newomics MnESI source and M3 multinozzle emitters interfaced with a Thermo QE UHMR mass spectrometer.
Application Note / English / Oct 2020

M3 Emitter for Microflow LC-MS Analysis of Native Antibodies

This application note describes a MnESI-MS platform for characterization of native monoclonal antibody (mAb), that achieves improved sensitivity and robustness, higher throughput, and better preservation of native state using the Newomics M3 emitters interfaced with a Thermo QE Plus mass spectrometer.
Application Note / English / Feb 2020

Microflow LC-Nanospray MS for Bottom-up Proteomics

This application note develops a MnESI-MS platform for bottom-up proteomics that achieves nanoflow sensitivity, excellent reproducibility, and high throughput by using Newomics M3 emitters interfaced with Thermo QE Plus and Lumos mass spectrometers.
Application Note / English / May 2019

Microflow LC-Nanospray MS for Targeted Proteomics

This application note develops a MnESI-MS platform for targeted proteomics that achieves enhanced sensitivity, high throughput, and robustness by using Newomics M3 emitters and a Thermo Quantiva Triple Quad mass spectrometer.
Application Note / English / May 2019

Newomics M3 Emitters for Shotgun Lipidomics

The application note illustrates a direct-infusion ESI-MS platform for shotgun lipidomics of human plasma sample, that achieves high throughput, high sensitivity and robustness using the Newomics M3 emitters interfaced with a triple quadrupole mass spectrometer.
Application Note / English / Dec 2019

A Versatile, Sensitive, and Robust Native LC-MS Platform for Intact Mass Analysis of Protein Drugs

Yan et. al., Regeneron, J. Am. Soc. Mass. Spectrum. 2020, 31, 10, 2171-2179
Very impressive work published recently by Regeneron. They describe the development of an integrated LC-MS platform for native protein drug characterizations, enabled by Newomics multinozzle emitters. In particular, the developed platform can tolerate a wide range of LC flow rates and high salt concentrations, which are critical for accommodating different nLC methods. The platform also achieves online charge-reduction native MS, which improves the characterization of both heterogeneous and labile biomolecules.

Robust and sensitive peptidomics workflow for plasma based on specific extraction, lipids removal, capillary LC setup and multinozzle ESI emitter

Rochat et. al., University of Lausanne, Talanta, 2020, 223, Part 1, 121617
An impressive publication by University of Lausanne. This article presents a new LC-MS workflow for determining native peptides in plasma at the low picomolar levels for clinical diagnosis, enabled by Newomics multinozzle emitters. The workflow has demonstrated utility in the first-time discovery of statherin-related peptides in blood with circadian oscillations.