MnESI Source
Microflow-nanospray Electrospray Ionization (MnESI) source that empowers Newomics award-winning M3 multinozzle emitters to multiply your mass spectrometry performance.
Microflow-nanospray Electrospray Ionization (MnESI) source that empowers Newomics award-winning M3 multinozzle emitters to multiply your mass spectrometry performance.
Enhanced Workflow
Built for Microflow-Nanoflow LCMS
Sensitivity
5 - 50x
more sensitive compared to conventional methods.
Robustness
CV ≤ 5%
from 300 plasma injections.
Throughput
< 1 min.
per sample. More than 60 samples per hour.
Product Details
Features
- Empowers Newomics multinozzle emitters for significantly improved LC/MS performance
- Delivers nanoflow sensitivity and lower detection limit
- Allows smaller sample amount
- Enables higher sample throughput and faster analysis speed
- Achieves high robustness and excellent reproducibility for complex matrix analysis
- Visualizes spray via high quality imaging system
Specifications
| Parameter | Specifications |
|---|---|
| Ionization Mode | Atmospheric pressure electrospray ionization (ESI), both positive and negative mode. |
| ESI Probe | Newomics M3 Emitter (refer to M3 Emitter specification) |
| Open or Closed System | Closed source |
| Flow Rate | Nano (20 – 1000 nL/min) |
| Spray Angle | Fixed angle of 25° |
| Spray Voltage | 0 – 6 kV |
| Sheath Gas Flow Setting | 0 – 30 |
| Auxiliary Gas Flow Setting | 0 – 20 (optional) |
| Translational Stage | Linear XY-axis manual stage, ±5 mm travel distance, 30 µm travel accuracy |
| Camera | 1.3M pixels, 20x ̶ 90x magnification rate, color CMOS sensor, up to 30 fps frame rate |
| Light Source | Cool white (9000K) 5 mm round LED light, luminous intensity 46400 mcd |
| Connection | Face seal, finger-tight 10-32 threaded fittings including Thermo nanoViper, IDEX MarvelX and MarvelXACT, Waters ZenFit, Phenomenex SecurityLink, VICI Cheminert C360IZR1, and nanoConnect |
| Unit Dimension | Width: 25 cm (9.8 in.) |
| Unit Weight | 3 kg |
| Model | Specifications |
|---|---|
| Catalog # IS-T01 | MnESI Source for Thermo Scientific New Generation Mass Spectrometers (e.g. Orbitrap Fusion Lumos, Eclipse, Exploris, and TSQ Altis Triple quadrupole mass spectrometers) |
| Catalog # IS-T02 | MnESI Source for Thermo Scientific Legacy Mass Spectrometers (e.g. Orbitrap Q Exactive mass spectrometers) |
| Catalog # IS-B01 | MnESI Source for Bruker Mass Spectrometers (e.g. maxis II QTOF, timsTOF Pro mass spectrometers) |
Performance Highlight
Newomics MnESI-MS platform streamlines analysis of native antibodies, antibody drug conjugates, and large protein complexes. It achieves static nanoflow sensitivity and excellent data quality, allows for automated processing and higher throughput via LC/MS, and provides better preservation of protein native state during the ESI process.
SEC-LC/MS analysis of GroEL tetradecaner protein complex using the MnESI-MS platform with M3 Emitter at the microflow of 5 µL/min.
Newomics MnESI-MS platform facilitates nucleic acid analysis in a negative mode. It delivers increased sensitivity and higher charge state which allows for detection of larger oligonucleotides with longer sequence, and better identification with more complete fragmentation (with a higher charge state).
Ion-Pair Reversed Phase (IP-RP) LC/MS Analysis of 75-mer tRNA using the MnESI-MS platform with M3 Emitter at microflow. MnESI platform attains improved sensitivity and higher charge state and comparable data quality vs. high-flow LC/MS method.
Newomics MnESI-MS platform accelerates bottom-up proteomics and quantitative proteomics. It attains nanoflow sensitivity, higher reproducibility, robustness, and throughput in the analysis of Hela cells and human plasma samples.
Comparison of sensitivity and reproducibility (peak area %RSD): MnESI-MS platform vs. nanoflow LC/MS performing PRM analysis of Promega peptide mixture spiked in 200 ng HeLa digest.
MnESI-MS platform with M3 emitter delivers > 40-fold sensitivity increase and comparable peak area reproducibility vs. conventional high flow LC/MS in targeted proteomics analysis of human plasma samples.
Newomics MnESI-MS platform allows for sensitive identification and quantitation of lipids in human plasma samples. It achieves increased sensitivity and assay reproducibility and allows hundreds of lipid species to be measured from a drop of blood.
MnESI-MS platform with M3 emitter achieves enhanced sensitivity (2.5-fold) vs. conventional nanoflow method using single nozzle emitters in shotgun lipidomics of human plasma samples.
Literature
Newomics Microflow-nanospray ESI-MS (MnESI-MS ) Platform
See the superior performance and features of our MnESI-MS platform based on Newomics award-winning multinozzle M3 emitter technology and the new MnESI source; and learn how the MnESI-MS platform benefits your LC/MS applications.
Brochure / English / Oct 2020
A Versatile, Sensitive, and Robust Native LC-MS Platform for Intact Mass Analysis of Protein Drugs
Yan et. al., Regeneron, J. Am. Soc. Mass. Spectrum. 2020, 31, 10, 2171-2179
Very impressive work published recently by Regeneron. They describe the development of an integrated LC-MS platform for native protein drug characterizations, enabled by Newomics multinozzle emitters. In particular, the developed platform can tolerate a wide range of LC flow rates and high salt concentrations, which are critical for accommodating different nLC methods. The platform also achieves online charge-reduction native MS, which improves the characterization of both heterogeneous and labile biomolecules.
Robust and sensitive peptidomics workflow for plasma based on specific extraction, lipids removal, capillary LC setup and multinozzle ESI emitter
Rochat et. al., University of Lausanne, Talanta, 2020, 223, Part 1, 121617
An impressive publication by University of Lausanne. This article presents a new LC-MS workflow for determining native peptides in plasma at the low picomolar levels for clinical diagnosis, enabled by Newomics multinozzle emitters. The workflow has demonstrated utility in the first-time discovery of statherin-related peptides in blood with circadian oscillations.
Webinars
Newomics at US HUPO 2021
A New LC-MS Platform for Clinical Proteomics
Na Parra
Senior Director of Marketing, Newomics
Recorded March 10th, 2021
Newomics Native MS Webinar
Newomics MnESI Platform – Multiply Your MS Performance
Na Parra
Senior Director of Marketing, Newomics
Rosa Viner
Program Manager, Thermo Fisher Scientific
Recorded November 19th, 2020
